Use Cases

Automated synthesis of gene fragments and clones from digital sequences. Using custom reagents; DNA fragments, clones and variant libraries can be built, amplified and assembled using Gibson or Golden Gate approaches in less than 21 hours.

Use Cases – BioXp 9600:

• High-fidelity Gene synthesis (300-1,800 bp)
• DNA cloning (up to 3,600bp into any vector),
• Cell-free DNA amplification,
• Custom variant DNA libraries (Up to 96 libraries per run, 300-800 bp size).

Use Cases– BioXp 3250:

• DNA cloning (up to 7,000bp into any vector),
• Cell-free DNA amplification,
• Custom variant DNA libraries,
• mRNA synthesis, up to 400-1,800 bases (capped and tailed synthetic mRNA).”

Use Cases

• De novo sequencing
• Targeted sequencing
• Plasmid Sanger sequencing
• CRISPR-Cas9 genome editing confirmation
• Cell line authentication
• Detection of SNPs

Use Cases

• DNA oligos
• Primer sequences

Use Cases

• Measure common viral titer backbones,
• Measure presence of residual DNA sequences
• Gene expression analysis
• Mutation analysis

Use Cases

• Gene expression analysis (TaqMan or SYBR Green assays
• Mutation detection
• Genetic variation analysis (SNP, CNV, insertions, deletions)
• High-resolution melting curve analysis
• Microsatellite analysis
• Gene regulation analysis (miRNAs, siRNAs, non-coding RNAs)
• Methylation analysis and bisulfite sequencing PCR.

Use Cases

• Assess sequence purity,
• Sequence quantity,
• Sequence integrity / degradation